Spatiotemporal cytometry-Simultaneous analysis of DNA replication and damage

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Spatiotemporal cytometry-simultaneous analysis of DNA replication and damage.

MANY subcellular phenomena occur in discrete spatial regions of the nucleus or cytoaplasm. Nuclear processes like DNA replication, transcription, or splicing take place in such small regions defined as foci or microfoci. Using the methods of modern optical microscopy these processes can be visualized in 3D space and time. Numerous labeling techniques are available for visualization of various c...

متن کامل

Analysis of chromatin integrity and DNA damage of buffalo spermatozoa

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

متن کامل

DNA Damage and Replication Checkpoints

■ Abstract DNA checkpoints play a significant role in cancer pathology, perhaps most notably in maintaining genome stability. This review summarizes the genetic and molecular mechanisms of checkpoint activation in response to DNA damage. The major checkpoint proteins common to all eukaryotes are identified and discussed, together with how the checkpoint proteins interact to induce arrest within...

متن کامل

Flow cytometry analysis of DNA damage and the evaluation of cytotoxicity of alkylating agents.

Monoclonal antibody (MAb) F7-26 generated against nitrogen mustard (HN2)-treated DNA (O.S. Frankfurt, Exp. Cell Res., 170: 369-380, 1987) reacted with regions of local DNA denaturation (distortion) induced by DNA alkylation. The relationship between immunoreactivity of cellular DNA with MAb F7-26 and cytotoxic effects of HN2, L-phenylalanine mustard (L-PAM), and 1,3-bis(2-chloroethyl)-1-nitroso...

متن کامل

Simultaneous analysis of DNA and cell surface immunoglobulin in human B-cell lymphomas by flow cytometry.

Cell suspensions prepared from human B-cell lymphomas were analyzed for cellular DNA and cell surface immunoglobulin (SIg) by flow cytometry. In addition to SIg-bearing neoplastic B-lymphocytes, these tumors contain nonneoplastic cells. Flow cytometry permitted selective analysis of DNA in subpopulations defined by the membrane antigen SIg and also permitted analysis of SIg of subpopulations de...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Cytometry Part A

سال: 2013

ISSN: 1552-4922

DOI: 10.1002/cyto.a.22399